Fig. 3

LACV infection induces higher cellular loss in weanling BCECs compared to adult BCECs. Weanling and adult BCECs, isolated separately from OB and cortex, were infected with LACV and imaged at × 10 objective magnification at 48 hpi (Scale bar = 100 μm). ZO1, LACV, and DAPI are shown in red, green, and blue channels respectively. Weanling OB BCECs (a–d) and adult OB BCECs (e–h) were mock-infected (a, e) or infected with LACV at MOIs of 0.1(b, f), 1 (c, g), and 10 (d, h). Weanling cortical BCECs (i–l) and adult cortical BCECs (m–p) were mock-infected (i, m) or infected with LACV at MOIs of 0.1(j, n), 1(k, o), and 10 (l, p). All images are representative of multiple areas from wells of two experiments (a single experiment set was used for setting the background fluorescence and image quantification). Arrows show areas of viral infection and loss of cells. Quantification of sum intensity of LACV to DAPI (q, r) area using several randomized images from each set. Quantification of each image is shown as a single symbol along with the mean (orange bar: mean of weanlings and green bar: mean of adults) for each group. For each viral inoculum dose, multiple unpaired t-tests were performed between weanling OB vs adult OB and weanling cortical vs adult cortical BCECs. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001