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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Age influences susceptibility of brain capillary endothelial cells to La Crosse virus infection and cell death

Fig. 4

LACV infection induced cellular morphology changes in BCECs. Cultures described in Fig. 3 were stained for DAPI (blue), LACV (green), and ZO1 (red) and imaged at × 40 objective magnification (Scale bar = 20 μm) to examine morphological changes of infected cells. Weanling OB BCECs (a–d) and adult OB BCECs (e–h) are shown. OB BCECs were mock-infected (a, e) or infected with LACV at MOIs of 0.1 (b, f), 1 (c, g), and 10 (d, h). Weanling cortical BCECs (i–l) and adult cortical BCECs (m–p) were mock-infected (i, m) or infected with LACV at MOIs of 0.1 (j, n), 1 (k, o), and 10 (l, p). Cell aggregation and disruption of ZO1 staining are shown by the arrow (d). Localization of ZO1 in an intracellular compartment is shown by an arrow (l). Combined counts of images from OB and cortex samples at 10 MOI inoculum doses of LACV and ZO1 stained samples showed an average of 3.7 ± 0.5 foci of cellular loss from 17 representative images of weanling BCECs. In adult BCEC, an average of 0.6 ± 0.3 foci of cellular loss were observed from 10 representative images. 0.2 ± 0.1 syncytia were observed in a total of 13 images of weanling BCECs whereas no syncytium was seen in a total of 12 images of adult BCECs

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