Fig. 7

Alteration of cerebrovascular tissue morphology in LACV induced vascular leakage. CLEM images of vibratome sectioned OBs from mock (a, b) and LACV-infected mice (c–f) injected with 100-nm fluorescent beads at 3 dpi. BCEC ultrastructure from mock-infected OB (a, b). LM image of vibratome-sectioned LACV-infected mice OB used to identify vascular leakage in blood vessels by TEM (c), with beads in brain parenchyma shown with white arrows (e). Ultrastructure of BCEC from areas of vascular leakage (d, f) showing compromised perivascular tissue region indicated by white arrowheads and altered BCEC morphology indicated by black arrows. TEM scale bar = 500nm, LM scale bar = 500 μm