Fig. 6

Effect of FPR2/ALX-dependent AMPK-mTOR pathway on Ac2-26-mediated in vitro BV2 microglial polarization. a Schematic diagram of the experimental design. b Representative photographs of double immunostaining and quantitative analyses of BV2 microglial polarization. M1-phenotype: iNOS⁺ (green) and Iba-1⁺ (red); M2-phenotype: Arg-1⁺ (green) and Iba-1⁺ (red). Scale bar = 100 μm. c qRT-PCR analyses of mRNA expressions of M1-phenotype markers (CD32 and iNOS) and M2-phenotype markers (CD206 and Arg-1). d ELISA analyses of the expressions of a pro-inflammatory cytokine IL-1β (M1-phenotype) and an anti-inflammatory cytokine IL-10 (M2-phenotype). e Representative western blotting bands and densitometric quantifications of phosphorylation of AMPKα and mTOR. Data were presented as the mean ± SD (n = 6/group) and were analyzed by one-way ANOVA followed by Bonferroni’s multiple comparison test. *p < 0.05, **p < 0.01, and ***p < 0.001, ^#p < 0.05, ^##p < 0.01, and ^###p < 0.001