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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Brain gray matter astroglia-specific connexin 43 ablation attenuates spinal cord inflammatory demyelination

Fig. 6

Microarray analysis and immunohistochemistry of Cx43 icKO and Cx43fl/fl microglia isolated from whole spinal cord and brain tissues during acute EAE. ac Heat maps comparing the expression of proinflammatory (a), anti-inflammatory (b), and chemokine-related (c) gene transcripts in RNA samples from microglia isolated from the spinal cords of Cx43 icKO and Cx43fl/fl mice (n = 3 per group) in the pre-immunized and peak phases (dpi 17) of EAE. d Immunostaining of lumbar spinal cord lesions by anti-Iba1 antibody in the pre-immunized and peak phases (dpi 17) of EAE. Insets indicate enlarged images in each figure. Scale bar: 200 μm. e Quantification of microglia circularity in the spinal cord in the preimmunized and peak (dpi 17) phases of EAE. The statistical significance of differences for each comparison was analyzed by one-way ANOVA, followed by Tukey’s post-hoc analysis. n = 4–5 for each group. ** P < 0.01, NS = not significant. f Immunofluorescence of lumbar spinal cord lesions in the peak (dpi 17) phase of EAE. The red color indicates i immunolabeling by anti-arginase-1 antibody, and the green color indicates immunolabeling by anti-Iba1 antibody. The dotted line on each figure indicates the boundary between the gray and white matter of the spinal cord. Insets indicate enlarged images in each figure. Scale bar: 200 μm. g Quantification of the Arg-1+ area fraction (%) in the spinal cord gray matter in the peak phase of EAE. h Quantification of the Iba1+ area fraction (%) in the spinal cord gray matter in the peak phase of EAE. Student’s t test was performed for statistical analysis. n = 5 for each genotype. NS = not significant

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