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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: NLRP3 ablation enhances tolerance in heat stroke pathology by inhibiting IL-1β-mediated neuroinflammation

Fig. 3

LPS plus heat exposure promoted NLRP3 inflammasome activation in murine hypothalamus by using Real time-PCR, Western Blot, and immunofluorescence staining assay. Hypothalamus was chosen to be the target tissue and four groups were analyzed accordingly. a Real time-PCR analysis of NLRP3 mRNA. b Real time-PCR analysis of pro-IL-1β mRNA. c Western Blot result of NLRP3-related proteins. d Band intensity analysis of NLRP3protein. e Band intensity analysis of IL-1βprotein. f Band intensity analysis of caspase-p10 protein. Band intensities were quantified by ImageJ software and the values of the target proteins were normalized to that of β-actin. The threshold cycle (CT) of target product was normalized to internal standard GADPH and calculated by using the comparative cycle threshold (ΔΔCt) method. Band intensities were quantified by Image J software and the values of the target proteins were normalized to that of β-actin. The results were expressed as the mean ± SEM. (n = 5 to 10). **p < 0.01, *p < 0.05

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Journal of Neuroinflammation

ISSN: 1742-2094