Fig. 4
Altered expression of tight junction (TJ) and adhesion molecules in the non-lymphoid peripheral tissues of IFN-I signal-incompetent mice. a–c The expression of TJ molecules in peripheral organs and brain of IFN-I signal-competent and -incompetent mice. The mRNA expression of TJ molecules in the liver (a), intestine (b), and brain (c) was evaluated by real-time qRT-PCR at the indicated days after JEV infection (5 × 106 ffu/mouse) via the footpad route. d The altered expression of adhesion molecules in IFN-I signal-incompetent mice. The mRNA levels of adhesion molecule including ICAM-1 and JAM-1 were determined by real-time qRT-PCR using total RNA extracted from liver, intestine, and brain at the indicated dpi. Data in bar graphs denote the mean ± SEM. Results are representative of one out of at least two individual experiments with four to five mice per group. Statistical significance *p < 0.05; **p < 0.01; ***p < 0.001 was assessed by an unpaired two-tailed Student’s t test