Fig. 1

miRNA profile in splenic NK cells of C57BL/6 mice subjected to MCAO. a Technology roadmap of NK cell isolation and profiling. Splenic NK cells isolated from C57BL/6 mice after MCAO. RNA sequencing to identify miRNA signatures and reverse validation by RT-PCR. b The heatmap shows the quantitative nCounter expression profiling of 270 miRNAs in NK cells from the spleens of sham-operated or MCAO mice on days 1, 3, and 7 after surgery. The relative abundance of transcripts is indicated by the color (red, high; green, low). Data were pooled from 15 mice in each group. c–e The bar graph shows selected miRNAs that were upregulated or downregulated in NK cells from the spleen on postoperative days 1, 3, and 7 in MCAO mice compared to control (sham-operated) mice. f Verification of miR-1224 expression in NK cells from the spleens and brains of sham-operated or MCAO mice on postoperative day 3. Data are representative of three independent experiments. n = 8 mice per group. **p < 0.01 by one-way ANOVA. Error bars represent the mean ± SEM