Fig. 3

miR-1224 inhibited the activation and cytotoxicity of NK cells from spleens after ischemic stroke. a Contour plots of flow cytometry show gating strategies and the expression of NK1.1, CD69, CD25, and IFN-γ from the spleens and brains of NOD-Prkdcscid IL2rg^−/− transgenic mice (lack of T, B, and NK cells) subjected to MCAO. b, c Bar graphs summarizing the effect of miR-1224 modulation on NK cell counts and the expression of CD69, CD25, and IFN-γ in splenic (b) and brain-infiltrating NK cells (c). Data are representative of three independent experiments. n = 11 mice per group. *p < 0.05, **p < 0.01 by two-way ANOVA. Error bars represent the mean ± SEM