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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: miR-1224 contributes to ischemic stroke-mediated natural killer cell dysfunction by targeting Sp1 signaling

Fig. 5

Downregulation of miR-1224 in NK cells by an inhibitor reduces the bacterial burden in the lungs of MCAO mice. a Schematic graph of the experimental design. Splenic NK cells isolated from C57BL/6 mice were transfected with miR-1224-mimics/inhibitor/negative control. miR-1224-regulated NK cells were then passively transferred via intravenous injection to NOD-Prkdcscid IL2rg−/− mice, followed by MCAO surgery. b Neurological deficits were assessed by the modified neurological severity score (mNSS), the corner test, and the rotarod test after NK cell transfer and MCAO surgery. n = 3~8 mice per group. p > 0.05 by two-way ANOVA. c Representative MR images and summarized data show stroke lesion volume after adoptive transfer of miR-1224-regulated NK cells; n = 3 mice per group. p > 0.05 by two-way ANOVA. d Lung tissues from MCAO mice treated with miR-1224-modulated NK cells were collected for bacteriological analysis 3 days after MCAO surgery. The bar graph illustrates the quantification of bacterial burden in the lungs of the indicated groups. Data are presented in colony-forming units (CFU) per lung tissue. n = 8 mice per group. *p < 0.05 by a two-tailed unpaired Student’s t-test. e NOD-Prkdcscid IL2rg−/− mice underwent adoptive transfer of NK cells transfected with an miR-1224 mimic plasmid or a control plasmid. Lung tissues were collected on post-MCAO day 3 for histological examination. The typical signs of bacterial burden (thickening of alveolar walls and neutrophilic infiltration) in MCAO mice were visible in lung sections stained with hematoxylin and eosin. Scale bars, 50 μm; insert, 25 μm. f The protein levels of IFN-γ in the serum of the control and mimic groups were measured by ELISA on post-MCAO day 3. n = 3~8 mice per group. *p < 0.05 by a two-tailed unpaired Student’s t-test. Data are representative of three independent experiments. Error bars represent the mean ± SEM

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