Fig. 4
From: Rutin prevents tau pathology and neuroinflammation in a mouse model of Alzheimer’s disease
Rutin attenuates tau pathology in Tau-P301S mice. A AT8 immunostaining for phosphorylated-tau in the brains of Tau-P301S mice and their WT littermates treated with rutin or vehicle (scale bar: 100 μm). B, C Quantification of AT8 immunostaining in the hippocampal CA1 (B) and DG (C) regions (n = 8 mice). D Western blot analysis of phosphorylated-tau (AT8), PP2A, and β-actin in the brain lysates of Tau-P301S mice and their WT littermates treated with rutin or vehicle. E, F Quantification of AT8 positive p-tau (E) and PP2A (F) in D (n = 8 mice). G Dot-blot analysis of OC-positive oligomers in the brain lysates of Tau-P301S mice and their WT littermates treated with rutin or vehicle. H Quantification of OC-positive tau oligomers (n = 8 mice). Data represent means ± SEM and were analyzed by one-way ANOVA with Tukey’s test (B–F, H). ***P < 0.001, ****P < 0.0001, ns, not significant