Fig. 3

Flow cytometry analyses of the hypothalamus (A, B, E), visceral fat pad (C, F), and peritoneal cavity (PEC, D, G) demonstrated macrophage migration following HFD. E An example of flow cytometry gating, indicating the presence of the CD45^high macrophage population that can be distinguished from the CD45^low microglia specifically in the hypothalami of HFD male mice, but not in female mice. These populations were quantified and presented as the % of all live cells in A (macrophages, CD11b+CD45^high) and B (microglia, CD11b+Cd45^low). C Quantification of F4/80+CD11b+ macrophages in the stromal vascular fraction of visceral adipose tissue following Ctr and HFD in males (left) and females (right). F An example of the flow cytometry gating strategy from visceral fat. D, G Peritoneal cavity (PEC) cells were analyzed by flow cytometry (gating example G) and the percent of F4/80+CD11b+ macrophages of all live cells are presented graphically in D, males on the left and females on the right. n=9–12 total, 3 repeats consisting of 3–4 mice per group. Statistical significance (*) between Ctr (gray bars) and HFD (black bars) were determined by Student’s T test followed by Tukey’s post hoc test