Fig. 5
Inhibition of D-DT suppressed production of PGE2 from astrocytes. a Western blot analysis of COX2 and mPGES-1 following astrocyte stimulation with 1 μg/ml recombinant D-DT in the presence or absence of 100 μM selective inhibitor 4-CPPC for 24 h. b, c Quantification data as shown in (a). Quantities were normalized to endogenous β-actin. d, e ELISA determination of PGE2 in supernatant and lysate following astrocytes stimulation with 1 μg/ml recombinant D-DT in the presence or absence of 100 μM 4-CPPC for 24 h. Experiments were performed in triplicates. Error bars represent the standard deviation (*P < 0.05)