Fig. 6
Effects of COX2 selective inhibitor NS398 on the astrocyte production of PGE2 in response to D-DT stimulation. a Western blot analysis of mPGES-1 following astrocyte treatment with 1 μg/ml recombinant D-DT in the presence or absence of 30 μM NS398 for 24 h. b Quantification data as shown in (a). Quantities were normalized to endogenous β-actin. c, d ELISA determination of PGE2 in supernatant and lysate following astrocytes stimulation with 1 μg/ml recombinant D-DT in the presence or absence of 30 μM NS398 for 24 h. Experiments were performed in triplicates. Error bars represent the standard deviation (*P < 0.05)