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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: D-dopachrome tautomerase activates COX2/PGE2 pathway of astrocytes to mediate inflammation following spinal cord injury

Fig. 7

Interference of CD74 receptor decreased D-DT-induced astrocyte production of PGE2. a–b Immunofluorescence showed colocalization of CD74 with S100β-positive astrocytes in the spinal cord. Rectangle indicates region magnified. Arrowheads indicate the positive signals. c Western blot analysis of CD74, COX2, and mPGES-1 following siRNA2 knockdown of CD74 receptor for 48 h, prior to stimulation with 1 μg/ml recombinant D-DT protein for 24 h. d–f Quantification data as shown in (c). Quantities were normalized to endogenous β-actin. g, h ELISA determination of PGE2 in supernatant and lysate following astrocytes transfected with CD74 siRNA2 for 48 h, followed by treatment with 1 μg/ml recombinant D-DT for 24 h. Experiments were performed in triplicates. Error bars represent the standard deviation (*P < 0.05)

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