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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: D-dopachrome tautomerase activates COX2/PGE2 pathway of astrocytes to mediate inflammation following spinal cord injury

Fig. 8

Inhibition of MAPKs inactivated COX2/PGE2 pathway of astrocytes. a Western blot analysis of COX2 and mPGES-1 following treatment with 1 μg/ml recombinant D-DT in the presence of 10 μM P38 (SB203580), 10 μM JNK (SP600125), or 10 μM ERK (PD98059) inhibitor for 24 h. b, c Quantification data as shown in (a). Quantities were normalized to endogenous β-actin. d, e ELISA determination of PGE2 in supernatant and lysate following astrocytes treatment with 1 μg/ml recombinant D-DT in the presence of 10 μM P38 (SB203580), 10 μM JNK (SP600125), or 10 μM ERK (PD98059) inhibitor for 24 h. Experiments were performed in triplicates. Error bars represent the standard deviation (*P < 0.05)

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