Fig. 9
Effects of selective inhibitor on the COX2/PGE2 pathway of astrocytes in response to co-stimulation of MIF and D-DT. a Western blot analysis of COX2 and mPGES-1 following astrocyte co-stimulation with 1 μg/ml recombinant MIF and/or equivalent D-DT in the presence or absence of 100 μM 4-IPP or 4-CPPC for 24 h. b, c Quantification data as shown in (a). Quantities were normalized to endogenous β-actin. d, e ELISA determination of PGE2 in supernatant and lysate following astrocyte co-stimulation with 1 μg/ml recombinant MIF and/or equivalent D-DT in the presence or absence of 100 μM 4-IPP or 4-CPPC for 24 h. Experiments were performed in triplicates. Error bars represent the standard deviation (*P < 0.05)