Fig. 8

Glycolytic inhibitor 2-DG significantly ameliorates MPTP-induced behavioral deficits in mice. a Schematic diagram of drug administration. Mice were injected intraperitoneally with 2-DG (400 mg/kg/day) or saline (NS) for sequential 10 days. On the fourth day, mice were injected intraperitoneally with MPTP (30 mg/kg/day) or saline (NS) for 7 days. Pole climbing test (b) and rotarod test (c) were conducted on the 11th day (n=10 mice per group). d Double immunostaining for TH and Iba-1 in the SN. Magnification, ×10, scale bar, 100 μm (left). Boxed rectangular regions were enlarged (right). Magnification, ×40, scale bar, 25 μm. e–f The number of TH-positive cells was quantified manually, and the optical density of Iba1 immunoreactivity in same sections were measured using ImageJ analysis software (n=5 mice per group). The data are presented as the means ± S.E.M. *p <0.05, **p <0.01, compared to the MPTP group. g TH protein expression in the striatum (STR) and SN was determined by Western blotting (left), and the relative protein levels were quantified by densitometric analysis (right, n=3 mice per group). The data are presented as the means ± S.D. *p <0.05, **p <0.01, compared to the MPTP group