Fig. 6

Neutrophil depletion abolished cordycepin conferred protection. a Representative images of flow cytometry analysis of neutrophils (Ly6G+) in the blood on 3 days after TBI. b Quantification of numbers of macrophages or neutrophils in the blood. n=4/group. c Representative images of Ly6G staining, white arrows indicate Ly6G+cells. Scale bar: 20 μm. d Quantification of numbers of Ly6G+cells in CTX and STR. n=5/group. e Representative images of IgG leakage on 3 days after TBI. Scale bar: 1 mm. f Quantification of IgG leakage area. n=6/group. g Representative CD16/Iba1 stained images in CTX and STR on 3 days after TBI, white arrows indicate CD16+Iba1+ cells. Scale bar: 20 μm. h Quantification of numbers of CD16+Iba1+ cells in CTX and STR. n=5/group. i Rotarod test and grid walking test results on 3 days after TBI. n=7~9/group. j Foot fault rate in grid walking test was correlated with the number of infiltration neutrophils (Ly6G⁺) in the brain. n=5/group. ^#p < 0.05, ^##p < 0.01, and ^###p < 0.001 vs. isotype group, *p < 0.05, **p < 0.01, and ***p < 0.001 vs. anti-Ly6G group, ns: no significance, as indicated