Fig. 8

Astrocytosis marker. GFAP DAB-stained representative right hemisphere brain sections of 11-month-old males a wt on St diet, b wt on HF diet, c THY-Tau22 on St diet, d THY-Tau22 on HF diet. Black- and white-framed inserts in left down and up right corners show magnified area in particular color frames. Scale bar for the whole section 500 μm, for inserts 200 μm. e A representative figure of analyzed areas: hippocampus (orange), cortex (purple). The red arrow point to cluster of reactive astrocytes. f Relative quantification at particular analyzed area (e) at right hemisphere brain sections of 7- and 11-month-old THY-Tau22 and wt mice (n = 24 sections per 4 mice). Mouse wt group on St diet was set as 100 %. g Western blots of hippocampal GFAP. h Quantification of g western blots of hippocampal GFAP in 7- and 11-month-old mice (n = 6). Mouse wt group on St diet was set as 100 %. The intensity of GFAP was related to particular β-actin intensity. All data are presented as mean ± SEM and were statistically analyzed using Mann-Whitney t test within each age and sex group (*). The age comparison of brain sections (F) was performed by mixed-effects analysis and Bonferroni’s post hoc test (^#). Statistical significance for all measurements: *p < 0.05; **p < 0.01; ***p < 0.001. DAB 3′,3′-diaminobenzidine, GFAP glial fibrillary acidic protein