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Table 2 Primary antibodies used at immunohistochemistry

From: Aging and high-fat diet feeding lead to peripheral insulin resistance and sex-dependent changes in brain of mouse model of tau pathology THY-Tau22

Primary antibody

Dilution

DAB staining

Manufacturer

GFAP rabbit pAb

1:200

1 min, 10 sec

Thermofisher, Rockford, IL, USA

Iba1 rabbit pAb

1:2000

3 mins

FUJIFILM Wako Pure Chemical Corp., Richmond, VA, USA

AT180 mouse mAb

1:400

-

Thermofisher, Rockford, IL, USA

  1. DAB 3,3′-diaminobenzidine, GFAP glial fibrillary acidic protein, Iba1 ionized calcium-binding adapter molecule 1
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Journal of Neuroinflammation

ISSN: 1742-2094