Fig. 4

Study design and validation of microglia Ager deletion. a Timing of Tamoxifen (TAM) administration and behavioral assessments of animals. b Quantification of Ager transcripts in primary CD11B⁺ isolates by RT-qPCR thirty days after TAM administration. c Representative images of IBA1 and RAGE staining in the ventral horn of SOD1^G93A mouse lumbar spinal cord at end-stage. Scale bar: 50 μm. d Quantification of IBA1 and RAGE overlap area. Mean ± SD. In b, N = 3 Ager^fl/fl Cx3cr1^+/+ mice and N = 4 Ager^fl/fl Cx3cr1^Cre/+ mice. In c, d, N = 6 SOD1^G93A Ager^fl/fl Cx3cr1^Cre/+ mice, N = 8 SOD1^G93A Ager^+/+ Cx3cr1^Cre/+ mice. Independent two sample two-sided t test. **p = 0.0031, *p = 0.0437