Fig. 5

Smek1 heterozygosity upregulates macrophage IL-1β expression in both the resting and activated states. a, b Flow cytometry of IL-1β⁺ macrophages with anti-mouse F4/80 and IL-1β antibodies (n = 3 control, n = 4 Smek1^-/+) (a); data are shown in the bar graph (b). c, d Mean fluorescence intensity of IL-1β in splenic IL-1β + macrophages (n = 3 control, n = 4 Smek1-/+) (c); data are shown in the bar graph (d). e qPCR analysis of the relative expression of macrophage cytokines in cultured mononuclear cells with and without lipopolysaccharide (LPS) treatment. Data are presented as the mean ± SEM and were analyzed by the two-sided unpaired t test; *P < 0.05; ***P < 0.001