Fig. 6

Smek1 is required for indoleamine 2,3-dioxygenase 1 (IDO1) maintenance in the immune system and CNS. a, b IFN-γ⁺ splenic and lymph node CD4⁺ Th1 cells were detected by flow cytometry (n = 7 in both groups) (left panel); data are shown in the bar graph (right panel). c qPCR analysis of the relative expression of T-bet and IFN-γ with and without anti-CD3&CD28 treatment. d Western blot and quantification analyses of the protein levels in splenic mononuclear cells with no stimulation and treatment with LPS and the anti-CD3&CD28 and anti-IFN-γ antibodies. e qPCR analysis of the mRNA level of IDO1 in the EAE brain (n = 9 in control, n = 8 in Smek1^-/+ mice). f qPCR analysis of the mRNA level of IDO1 in the EAE spleen (n = 9 in control, n = 8 in Smek1^-/+ mice). g Western blot and quantification analyses of the IDO1 protein levels in the EAE brain. h Western blot and quantification analyses of IDO1 protein levels in the EAE spleen. i Representative western blot analysis of IDO1 expression level in EAE brain and spleen (n = 8 in each group). Data are presented as the mean ± SEM and were analyzed by the two-sided unpaired t test; *P < 0.05; **P < 0.01; ***P < 0.001