Fig. 3

Validation of TBI-induced gene expression in microglia by dual in situ hybridization and immunofluorescence. A–D. Representative confocal images demonstrating TBI-induced, microglial Clec7a expression. Seven days post-FPI, Clec7a co-localized with microglial marker, IBA1 in A cortex, B corpus callosum, C hippocampus, and D thalamus (Scale bar, 50 um). E–L Quantification of number of IBA1+ microglia, and IBA1+ cells expressing Clec7a in sham vs FPI mice in E, I cortex; F, J corpus callosum; G, K hippocampus; and H, L thalamus. Error bars depict mean ± SEM. Statistical analysis performed by two-sample, unpaired t tests. ***p < 0.001; *p < 0.05. n = 4 sham, 6 FPI mice