Fig. 3

NLPR3 knockout protected against neuroinflammation and neurodegeneration from DSS administration. A Immunofluorescence staining of neurons and microglia in the cortex (25× water immersion objective). B Comparison of neuronal (i) and microglia (ii) numbers among control WT, DSS-fed WT, NLRP3 KO, and DSS-fed NLRP3 KO mice (average of four fields/slice, five slices per mouse, 6 mice per group). C Immunofluorescence staining of neurons and microglia in the hippocampus (CA1 area, 25× water immersion objective). D Comparison of neuronal (i) and microglial (ii) number among treatment groups (CA1 area, average of two fields/slice, five slices per mouse, 6 mice per group). E Immunofluorescence staining of MAP2 (25× water immersion objective) in the cortex. F Comparison of MAP2 immunostaining intensity in the cortex among treatment groups (average of four fields/slice, five slices per mouse, 6 mice per group). G Immunofluorescence staining of MAP2 in the hippocampus (CA1 area, 25× water immersion objective). H Comparison of MAP2 staining intensity in the hippocampus among treatment groups (average four fields/slice, five slices per mouse, 6 mice per group). Each dataset is expressed as mean ± SD. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001. n = 6 mice