Fig. 5

Colitis disrupted the polarity of astrocytic AQP4 distribution in WT but not NLRP3 KO mice. A Representative images of astrocytes and AQP4 expression in the cortex (Left 3 rows: 25× water immersion objective; right row: expanded images (3×) of regions in the white boxes). B Comparison of astrocyte number (i), AQP4 expression intensity (ii), and AQP4 polarity (iii) in the cortex among control WT, DSS-fed WT, NLRP3 KO, and DSS-fed NLRP3 KO mice. C Representative images of astrocytes and AQP4 expression in the hippocampus (Left three rows: 25× water immersion objective; right row: expanded images (3×) of regions in the white boxes). D Comparison of astrocyte number (i), AQP4 expression intensity (ii), and AQP4 polarity (iii) in the hippocampus among treatment groups. Each dataset is expressed as mean ± SD. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001. n = 6 mice