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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Fundamentally different roles of neuronal TNF receptors in CNS pathology: TNFR1 and IKKβ promote microglial responses and tissue injury in demyelination while TNFR2 protects against excitotoxicity in mice

Fig. 6

Preconditioning protection against KA seizures and hippocampal neuron survival is reduced in nTNFR2KO mice. (A) Mean seizure scores recorded every 5 min after acute i.p. injection of 24 mg/kg KA in nTNFR1KO (n = 4; gray circles) and TNFR1ff control (n = 4; black circles) mice. (B) Mean seizure scores recorded every 5 min after acute i.p. injection of 20 mg/kg KA in nTNFR2KO (n = 5; gray circles) and TNFR2ff control (n = 5; black circles) mice, or after preconditioning with i.p injection of 15 mg/kg KA followed after 24 h by i.p. injection of 20 mg/kg KA in TNFR2KO (n = 4; gray squares) and TNFR2ff control (n = 4; black squares) mice. (C) Cresyl violet (Nissl) staining of brain coronal paraffin sections from mice shown in (B) taken 5 days after acute KA administration in preconditioned (PC+acute) mice. (Ci) Low power photographs showing the variable localization of pyknotic dead neurons in the CA1, CA2, and CA3 regions of the hippocampus from nTNFR2KO mice (arrowheads). (Cii) Quantification of pyknotic dead neurons in mice showing an affected CA3 region, as mean % dead cells/ set area of CA3 (3.3 mm × 1.7 mm). Scale bar: 200 μΜ. Results are representative of one or two (acute KA in nTNFR2KO and TNFR2ff control mice) independent experiments and are presented as mean values ± SEM. Statistical significance after comparisons between acute KA versus preconditioning KA in each genotype is shown by one-way ANOVA with Bonferroni’s test for each time point. *p ≤ 0.05

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