Fig. 7

Microglia influence astrocytic transformation via the GAS6/AXL axis. (A) Immunofluorescence staining of GAS6 (red) with IBA1, GFAP, or NeuN (green) after TBI; nuclei were stained by DAPI (blue). (B) GAS6 level was suppressed by minocycline administration at 3 dpi. *p < 0.05 **p < 0.01 versus sham, ^##p < 0.01 versus vehicle by one-way ANOVA. (C) ABCA1 level was suppressed after minocycline treatment at 3 dpi. *p < 0.05, **p < 0.01 versus sham, ^##p < 0.01 versus vehicle by one-way ANOVA. (D) The effect of GAS6 specific targeted siRNA or scramble siRNA after transfected into BV2 cells. *p < 0.05 by two-tailed Student’s t-test. (E) Brief workflow of in-vitro cell crosstalk analysis. (F) BV2 supernatant enhanced primary astrocyte phagocytosis after 24 h co-culture, as detected by flow cytometry. **p < 0.01 by two-tailed Student’s t-test. (G) Flow cytometry analysis of BV2 supernatant-induced astrocytic phagocytosis showed it was abolished after GAS6 siRNA transfection. **p < 0.01 versus scramble by one-way ANOVA