Fig. 1

Expression level of progranulin in eyes of a choroidal neovascularization (CNV) model mouse. a Immunofluorescence staining of the laser-injured eye of C57BL/6J mice (8 weeks old) at 0, 1, 3, 5, 7, and 14 days after the photocoagulation with anti-PGRN (green) and anti-Iba-1 (red) antibodies. Nuclei were stained with Hoechst 33342 (blue). Phase contrast images are also shown. The yellow dotted line shows the area that was counted. Scale bar: 50 μm. b Enlarged images at 3 days after laser coagulation. Scale bar: 10 μm. c Graph of the ratio of PGRN⁺ cells in Iba-1⁺ cells around CNVs. Data are presented as percentages. Expression of PGRN was observed in 65–80% of Iba-1⁺ cells in CNV lesions. d, e Quantitative analysis of the number of Iba-1⁺ cells and PGRN⁺Iba-1⁺ cells in the subretinal area at 0, 1, 3, 5, 7, and 14 days after photocoagulation. Data are the means ± SEMs, (n = 5). ^*P <0.05, ^**P <0.01 vs. Control (d: one-way ANOVA followed by Dunnett’s test, e: Dunn-Bonferroni post hoc method following significant Kruskal-Wallis tests)