Fig. 3

Larger size laser-induced CNV lesion and higher number of Iba-1⁺ cells in the progranulin knockout mice. a Representative microscopic images of FITC-dextran angiogram and immunostaining of Iba-1 of RPE-choroid flat mounts from Grn^+/+, Grn^+/−, and Grn^−/− mice (9-12 weeks). White dotted line shows CNV area. Scale bars: 50 μm. b. Quantification of the mean size of the CNV areas. c Quantification of the number of Iba-1⁺ cells around the CNVs (Grn^+/+, n = 9; Grn^+/−, n = 14; and Grn^−/−, n = 10). Data are the means ± SEMs. ^*P <0.05, ^**P <0.01 vs. Grn^+/+; ^#P <0.05, ^##P <0.01 vs. Grn^+/− (b: Dunn-Bonferroni post hoc method following significant Kruskal-Wallis tests, c: one-way ANOVA followed by Tukey’s test)