Fig. 2

Effect of TATκ-GFP-CDKL5 administration on microglial cell activation in Cdkl5 KO mice. A Quantification of AIF-1-positive cells in hippocampal (Hippo) and somatosensory cortex (Cortex) sections from 6-month-old wild-type (+/Y n=2) mice and Cdkl5 -/Y mice treated with TATκ-GFP (n= 5) or TATκ-GFP-CDKL5 (n= 5) protein as described in [36]. B Mean microglia cell body size in the hippocampus and somatosensory cortex of Cdkl5 KO mice as in A. Data in A and B are presented as means ± SEM. * p<0.05; ** p<0.01; ***p<0.001 (Fisher’s LSD after one-way ANOVA). C Examples of cortical sections processed for AIF-1 immunostaining of a Cdkl5 -/Y mouse treated with TATκ-GFP or TATκ-GFP-CDKL5 as in A