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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Inhibition of microglia overactivation restores neuronal survival in a mouse model of CDKL5 deficiency disorder

Fig. 5

Effects of luteolin treatment on neuroinflammatory gene expression in microglial cells of Cdkl5 KO mice. A Real-time qPCR analysis of interleukin 1 beta (IL-1β), interleukin 6 (IL-6), and tumor necrosis factor alpha (TNFα) gene expression in microglial cells isolated from the brain of 3-month-old Cdkl5 +/+ (n=6) and Cdkl5 +/- (n=5) mice and 7-day luteolin-treated Cdkl5 +/- (n=6, Lut) mice. B Expression of CX3C chemokine receptor 1 (CX3CR1) and allograft inflammatory factor 1 (AIF-1) in microglial cells isolated from the brain of mice as in A. Data are given as fold change in comparison with microglial cells from Cdkl5 +/+ mice. * p<0.05; ** p<0.01; (Fisher’s LSD test after one-way ANOVA). C, D Western blot analysis of P-STAT3 (Tyr 705), STAT3, AIF-1, and GAPDH levels in microglial cells isolated from the brains of untreated Cdkl5 mice (+/+ n=4, +/- n=4) and 7-day luteolin-treated Cdkl5 +/- mice (n=6). Histograms show P-STAT3 protein levels normalized to corresponding total STAT3 protein levels (C, left panel), STAT3 levels normalized to GAPDH (C, right panel), and AIF-1 levels normalized to GAPDH (D). E Example of immunoblots from the same experimental group as in C. The results in C and D are expressed as percentages of protein levels in Cdkl5 +/+ microglial cells. Values are represented as means ± SEM of three technical replicates from the same sample; each sample has been obtained by mixing microglial cells purified from the brains of untreated Cdkl5 +/+ and Cdkl5 +/- mice (n=4) and 7-day luteolin-treated Cdkl5 +/- mice (n=6). ***p < 0.001 (Fisher’s LSD test after one-way ANOVA)

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