Fig. 6

Effect of luteolin treatment on survival and maturation of postmitotic granule neurons in Cdkl5 KO mice. A Examples of sections processed for DCX immunostaining from the dentate gyrus (DG) of an untreated wild-type (+/+), a heterozygous Cdkl5 KO female (+/-), and a 7-day luteolin-treated heterozygous Cdkl5 KO female (10 mg/Kg; +/- Lut 7d) mouse. B Number of DCX-positive cells in the DG of untreated Cdkl5 +/+ (n=6) and Cdkl5 +/- (n=6) mice, and 7-day luteolin-treated Cdkl5 +/- mice (Lut +, n=4). Data are expressed as number of DCX positive cells per 100 μm of granule cell layer. C Number of Ki-67-positive cells in the DG of mice as in B. D Mean total dendritic length of CA1 pyramidal neurons of mice as in B. Values in B, C, and D are represented as means ± SEM. *p< 0.05, **p< 0.01 (Fisher’s LSD test after one-way ANOVA). E, F Western blot analysis of BDNF and GAPDH levels in somatosensory cortex homogenates from untreated Cdkl5 mice (+/+ n=4, +/- n=4) and 7-day luteolin-treated Cdkl5 +/- mice (+/- Lut7d, n=4). Examples of immunoblot in E. Histogram in F shows mature BDNF protein levels normalized to GAPDH. Data are expressed as a percentage of untreated Cdkl5 +/+ mice. Values are represented as means ± SEM of two technical replicates; *p<0.05;**p < 0.01 (Fisher’s LSD test after one-way ANOVA)