Fig. 9

Effect of luteolin treatment in middle-aged Cdkl5 +/- mice. A Schedule of treatment. Middle-aged (11-month-old) Cdkl5 mice were treated with vehicle or luteolin for 7 days. Mice were sacrificed 1 day after the end of treatment. B Mean AIF-1-cell body size of microglial cells in the hippocampus of vehicle-treated (+/+ n=4; +/- n=4) and luteolin-treated (+/- n=4, Lut) middle-aged Cdkl5 mice. C, D Quantification of Hoechst-positive cells (C) and NeuN positive cells (D) in CA1 layer of hippocampal sections from mice treated as in C. E Number of cleaved caspase-3 positive cells in the hippocampus of middle-aged (11-month-old) Cdkl5 mice treated as in A. Data are given as a percentage of NMDA-treated Cdkl5 +/+ mice. The results in B, C, D, and E are presented as means ± SEM. * p<0.05; ** p<0.01; ***p<0.001 (Fisher’s LSD test after one-way ANOVA)