Fig. 2

NLRP3 inhibition with MCC950 prevents microglia morphology shift after SAH. A–C Representative images of Iba1-stained (red) cerebral cortex in A sham, B SAH + vehicle, and C SAH + MCC950 groups with DAPI nuclear counterstain (blue). Scale bars = 50μm, all images captured with 40× objective. Inset: Enlarged images of individual cell bodies. D Microglia morphology analysis via quantification of ramification endpoints per cell. E Total number of Iba1+ cells per high-powered field as a measurement of microglial burden. Data presented as mean ± SEM, n = 5–6 per group for all data, ** p < 0.01 compared to sham surgery group by Kruskal-Wallis test with Dunn’s multiple comparisons test