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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Differential effects of the cell cycle inhibitor, olomoucine, on functional recovery and on responses of peri-infarct microglia and astrocytes following photothrombotic stroke in rats

Fig. 3

Effect of olomoucine treatment on immunolabelling for Iba1, CD68, and Ki67 at 3 days after stroke. A The top panel shows Iba1 immunolabelling in a coronal section including the infarct and surrounding cortex and equivalent tissue in the contralateral hemisphere. The rectangles indicate the location of the images in the lower panels, which have been processed for analysis of the area fraction of Iba1 immunolabelling and circularity of Iba1-positive cells. The scale bar represents 200 μm. Representative unprocessed images are shown in Additional file1; Fig. S2. B, C The effect of treatment on the area fraction of Iba1 immunolabelling (B) and circularity of Iba1-immunolabelled cells (C). For both measures, there was a significant effect of treatment (p < 0.05) and distance from the lesion (p < 0.01) in the peri-infarct tissue with no significant interaction. There was also a significant effect of treatment but not location in the contralateral hemisphere. D. The effect of treatment on CD68-positive cells. In the peri-infarct tissue, there was a significant effect of distance (p < 0.05) but not treatment (p = 0.065), whereas the contralateral cortex showed a significant effect of treatment (p < 0.01) but not tissue location. E The effect of treatment on Ki67-positive cells. This marker differed significantly with distance from the lesion in peri-infarct tissue (p < 0.05) Olomoucine did not significantly affect the numbers of Ki67-positive cells in either hemisphere. Results in B to E are presented as mean ± SD (n = 6 rats per treatment group) with data for each hemisphere analyzed using two-way analysis of variance. *p < 0.05; **p < 0.01 for the effect of olomoucine treatment. #p < 0.05; ##p < 0.01 for the effect of distance from the infarct or of location in the contralateral cortex

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