Fig. 6

Impact of HIV-1 infection on ACE2 and TMPRSS2 expression in pericytes. Human primary pericytes were either mock-infected or infected with HIV-1 as in Fig. 5 and the expression levels of ACE2 and TMPRSS2 were measured by q-PCR (A and D, respectively), immunoblotting (B and E, respectively), and immunostaining (C and F, respectively). GAPDH was used as a housekeeping gene and α-tubulin as a loading control. Graphs indicate the mean ± SD from three independent experiments; n = 4 per group; scale bars, 30 μm