Fig. 1

A decline in proliferation rate constrains IBA1⁺ cell numbers as CSF1 OE mice age. Cells were quantified in both the brainstem and midbrain for both control (CON, white bars) and CSF1 OE (OE, grey bars) mice. A Quantification of the percent of cells that are IBA1⁺. B Quantification of the percent of IBA1⁺ cells that are proliferating (Ki67⁺). C Quantification of the percent of IBA1⁺ cells that are apoptotic (TUNEL⁺ IBA1⁺). ND = none detected. ns = non-significant (p < 0.05); *p < 0.05; **p < 0.01; ***p < 0.001; and ****p < 0.0001 by unpaired, two-tailed t test with the exception of brainstem 6-month apoptotic cells which was analyzed by Wilcoxon signed-rank test