Table 1 Antibodies and dilutions and utilized in this study
From: Microglial responses to CSF1 overexpression do not promote the expansion of other glial lineages
Primary antibodies | ||||||
Target | Host species | Catalog # | Manufacturer | Dilution | RRID | Â |
IBA1 | Rabbit | 019-19741 | Wako | 1:200 | AB_839504 | Â |
IBA1 | Goat | ab48004 | Abcam | 1:150 | AB_870576 | Â |
ARG1 | Rabbit | ab91279 | Abcam | 1:200 | AB_10674215 | |
MRC1 (CD206) | Rabbit | ab64693 | Abcam | 1:1000 | AB_1523910 | Â |
Ki67 | Mouse | 550609 | BD Biosciences | 1:200 | AB_393778 | Â |
OLIG2 | Rabbit | AB9610 | Millipore Sigma | 1:150 | AB_570666 | Â |
GFAP | Chicken | ab4674 | Abcam | 1:200 | AB_304558 | Â |
GFAP | Rabbit | ab7260 | Abcam | 1:100 | AB_305808 | Â |
CSF1 | Goat | AF416 | R&D systems | 1:25 | AB_355351 | Â |
PDGFRA | Goat | AF1062 | R&D systems | 1:200 | AB_2236897 | Â |
Secondary antibodies | ||||||
Target | Host species | Catalog # | Manufacturer | Dilution | RRID | Conjugation |
Anti-mouse | Goat | ab97239 | Abcam | 1:200 | AB_10680851 | FITC |
Anti-rabbit | Donkey | ab150076 | Abcam | 1:200 | AB_2782993 | Alexa 594 |
Anti-chicken | Goat | ab150175 | Abcam | 1:200 | AB_2732800 | Alexa 647 |
Anti-goat | Donkey | ab150129 | Abcam | 1:250 | AB_2687506 | Alexa 488 |
Anti-goat | Donkey | A21447 | Invitrogen | 1:200 | AB_141884 | Alexa 647 |
Anti-mouse | Horse | MKB-2225 | Vector Laboratories | 1:250 | AB_2336564 | biotina |