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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: VMP1, a novel prognostic biomarker, contributes to glioma development by regulating autophagy

Fig. 4

VMP1 knockout inhibits cell proliferation and induces cell death. A A KEGG signaling pathway enrichment analysis of differentially expressed genes (DEGs) between the VMP1 high and low groups. The intensity of the color represents the − log10 adjusted P-value, and dot sizes show the number of DEGs enriched in the corresponding pathway. B GSEA revealed that genes in the VMP1 high group were enriched in cancer hallmark pathways. Gene sets with normalized enrichment score (NES) > 1 and nominal P-value (NOM P-val) < 0.05 were considered significant. C Schematic diagram illustrating the CRISPR-Cas9-mediated VMP1 editing system and western blotting to confirm the decrease in VMP1 expression. D Cell growth in the wild-type group, VMP1 sgRNA1 group (VMP1 KO1), and VMP1 sgRNA2 group (VMP1 KO2). E, F Results of a clonogenic assay. Red circles represent the clones being counted. G, H Visualization of DNA replication by EdU incorporation. Cell nuclei stained in red represent DNA replication. I Annexin V-FITC and PI staining to determine the percentage of apoptotic cells. J Cell cycle analysis. P-values were determined via Student’s t-test (*P < 0.05; **P < 0.01; ***P < 0.001)

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