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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: High-parameter cytometry unmasks microglial cell spatio-temporal response kinetics in severe neuroinflammatory disease

Fig. 1

Microglia cannot be reliably distinguished by standard macrophage or microglia-specific markers during severe inflammation. a, b FACs plots showing the expression of CD45 vs CD11b (a) and Ly6C vs CD11b (b) on live, Ly6G cells from murine brains at day 0, 3, 5, 6 and 7 post WNV-infection. Red circles show microglia in dpi 0 and 3 brains, and a mixed population of microglia and macrophages in dpi 5-7 brains. c tSNE plots showing the infiltration and accumulation of Ly6Chi cells from dpi 5-7. d Histograms showing the downregulation of Ly6C, and upregulation of CD45, CD11b and CX3CR1 on Ly6Chi macrophages from dpi 5-7. e FACs plots showing the expression of ‘microglia-specific’ marker TMEM119 on live, Ly6G cells in mock-infected murine brains digested with and without collagenase and DNase. Values on FACs plots represent the frequency of live, Ly6G, TMEM119+ cells. f Histogram showing the lack of expression of TMEM119 on live, Ly6G cells from mock-infected brains processed with collagenase and DNase. Fluorescence minus one (FMO) for TMEM119 is shown in grey. g-h FACs plots showing the expression of ‘microglia-specific’ markers TMEM119 (g) and P2RY12 (h) on live, Ly6G cells at WNV dpi 0 and 7. All data shown is representative of at least two independent experiments

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