Fig. 4

Dimensionality reduction analysis reveals four microglia subsets that adopt distinct phenotypes in WNE. a-c tSNE plots clustered on live myeloid cells (UVLD⁻ CD11b⁺ CD45⁺) from WNV dpi 0 and 7 murine brains. Mock-infected and WNV dpi 7 cells overlaid onto tSNE plots (a). Annotation of myeloid populations gated and overlaid onto tSNE plots representing mock-infected (b) and infected (c) brains. tSNE plots showing the relative intensity of selected markers/parameters in mock-infected (d) and infected brains (e). f Heatmap showing the expression of selected markers in P2RY12^hi CD86⁺, P2RY12^lo CD86⁻, P2RY12^lo CD86⁺ and P2RY12^hi CD86⁻ microglia and unstained (uns.) brain cells. g MFI of TMEM119 on 4 microglia populations in mock-infected and WNV-infected brains. h Comparison of P2RY12 and TMEM119 MFI on four microglia populations in WNV-infected brains. Data is representative of at least two independent experiments and presented as mean ± SEM, with four mice per group. *P < 0.0332, **P < 0.0021, ***P < 0.0002, ****P < 0.0001, two-way ANOVA with a Šídák’s multiple comparisons test