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Table 1 Primary antibodies

From: Celastrol exerts a neuroprotective effect by directly binding to HMGB1 protein in cerebral ischemia–reperfusion

Primary antibodies Host Dilution ratio Supplier
MAP2 Mouse 1:500 (IF) Abcam, United States
β-tubulin Rabbit 1:500 (IF) Abcam, USA
β-actin Mouse 1:5000 (WB) Affinity Biosciences, USA
PCNA Rabbit 1:3000 (WB) Proteintech Group, USA
NF-κB p65 Mouse 1:1000 (WB)
1:500 (IF)
Proteintech Group, USA
HSP70 Rabbit 1:1000 (WB)
1:500 (IF)
Abcam, USA
HMGB1 Rabbit 1:1000 (WB)
1:500 (IF)
Abcam, USA
RAGE Rabbit 1:1000 (WB) Proteintech Group, USA
TLR4 Rabbit 1:1000 (WB) Proteintech Group, USA