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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Aicardi-Goutières syndrome-associated mutation at ADAR1 gene locus activates innate immune response in mouse brain

Fig. 1

ADAR1 K999N mouse preparation. A Using CRISPR/Cas9 technology, a single nucleic guanosine (G) to thymidine (T) mutation was introduced into the mouse genome that codes the K948N mutation in mice, equivalent to the K999N mutation found in AGS patients. The genome G > T replacement was confirmed by Sanger sequencing in the founder and progenies.B This mutation does not alter the level of gene transcription or ADAR1 protein expression in the brain, as shown by real-time RT-PCR (n = 5, p = 0.905) and C Western blot analysis. ADAR1 protein levels in 4 wt and 5 mutant mouse samples are shown. D PCR genotyping was optimized to distinguish the wt and mutant alleles. The specific amplifications for G > T mutant and wt alleles are as indicated. The Wilcoxon rank-sum test was used to determine the statistical difference for the analysis in B and D

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