Fig. 1

Effect of rA2 on peripheral neutrophils after TBI. A Timeline of the experimental procedures for the pathological evaluation. B Representative gating strategy of peripheral neutrophils (CD11b⁺Ly6G⁺) and their expression of IL-6, TNFα, and IL-10. All gates were set using FMO controls. C Cell counts of peripheral neutrophils of sham, TBI, and TBI + rA2 mice at 24 h after TBI, n = 8. D Respective bar graphs show the cell counts of IL-6, TNFα, and IL-10 in peripheral neutrophils of sham, TBI, and TBI + rA2 mice at 24 h after TBI, n = 8. E RT-qPCR analysis of mRNA levels of IL-1β and TNFα in neutrophil that isolated from sham, TBI, and TBI + rA2 mice at 12 h after TBI, n = 4. F RT-qPCR analysis of mRNA levels of IL-1β and TNFα in monocytes that isolated from sham, TBI, and TBI + rA2 mice at 12 h after TBI, n = 3. Data are expressed as mean ± SEM; *p < 0.05 compared to sham; ^#p < 0.05 compared to TBI