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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Inhibition of heat shock protein family A member 8 attenuates spinal cord ischemia–reperfusion injury via astrocyte NF-κB/NLRP3 inflammasome pathway

Fig. 1

HSPA8 is highly expressed in rats after SCII. A The relative protein level of HSPA8 in spinal cord tissues was detected after 0 h, 12 h, and 24 h of reperfusion via western blot assay. B Double immunofluorescence of HSPA8 and GFAP in spinal cord tissues was performed. Scale bar = 50 μm. C The immunofluorescence intensity of HSPA8 was quantified. These data are representative of three experiments and are shown as the means ± SEM. Data were shown as means ± SD of three independent experiments. N = 6 rats per group. Statistical analysis was performed by one-way ANOVA followed by Turkey’s for multiple comparisons. *p < 0.05 vs. sham. SCII, spinal cord ischemia–reperfusion injury; GFAP, glial fibrillary acidic protein

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