Fig. 7

Ultrastructural analysis of the dorsal hippocampus CA1 of mice depleted in microglia and infected with ZIKV. a The left picture shows immunogold particles in the cytoplasm, endosome, and mitochondria of a perivascular cell in the stratum radiatum of untreated mice on day 7 post-infection. The right picture shows immunogold particles in the endosome of an astrocyte in the stratum radiatum of PLX5622-treated mice on day 7 post-infection. b The left picture shows nanogold particles in the mitochondria and at the nuclear membrane of a perivascular cell in the stratum lacunosum-moleculare of untreated mice on day 7 post-infection. The right picture shows immunogold particles in the cytoplasm and endosome of an astrocyte in the stratum lacunosum-moleculare of PLX5622-treated mice on day 7 post-infection. Insert in the right corner of each picture shows the subcellular localization of nanogold particles. c Pictures illustrate differences in content of endosomes with nanogold particles in different cell types: from left to right, neuron, perivascular cell, and astrocytes (two pictures at the right) from the stratum lacunosum-moleculare. Endosomes are pseudo-colored to distinguish empty endosomes (light blue) from endosomes with digested content (light purple). On the example pictures, cell types are identified by a capital letter: A, astrocyte; EC, endothelial cell, and PC, perivascular cell. Astrocytic intermediate filaments are also identified by a white asterisk (*). Nanogold particles, which stain ZIKV antigens, are highlighted by yellow arrowheads. Scale bars on the picture are equivalent to 1 µm for the whole picture and for the insert