Microglia are involved in phagocytosis and extracellular digestion during Zika virus encephalitis in young adult immunodeficient mice

Table 2 Density and distribution of Iba1⁺/TMEM119⁺ microglial cells and infiltrating Iba1⁺/TMEM119⁻ myeloid cells in both layers of the dorsal hippocampus CA1

Parameters	*Stratum radiatum*			*Stratum lacunosum-moleculare*
Parameters	Day 0 Mean ± SEM	Day 7 p.i. Mean ± SEM	Day 10 p.i. Mean ± SEM	Day 0 Mean ± SEM	Day 7 p.i. Mean ± SEM	Day 10 p.i. Mean ± SEM
Iba1⁺/TMEM119⁺microglial cells
Density (cells/mm²)	211.3 ± 4.8	206.2±20.4	218.0 ± 13.7	232.3 ± 11.0	220.9 ± 26.3	275.5 ± 23.8
Spacing index (a.u.)	0.474 ± 0.007	0.453±0.013	0.480 ± 0.018	0.484 ± 0.016	0.477 ± 0.014	0.497 ± 0.010
NND (μm)	47.75 ± 0.66	48.14±1.94	48.00 ± 1.49	45.80 ± 2.08	47.79 ± 3.04	43.97 ± 1.62
Number of clusters per animal	0.000 ± 0.000	0.150±0.081	0.333 ± 0.109	0.000 ± 0.000	0.150 ± 0.081	0.333 ± 0.109
Infiltrating Iba1⁺/TMEM119^-myeloid cells
Density (cells/mm²)	0.6393 ± 0.5245	0.1873 ± 0.1186	1.030 ± 0.664	3.510 ± 1.469	2.454 ± 0.786	4.843 ± 1.808
Ratio over Iba1⁺ cells (%)	0.2817 ± 0.2086	0.0940 ± 0.0604	0.4300 ± 0.2764	1.288 ± 0.510	1.102 ± 0.330	1.672 ± 0.458

a.u., arbitrary unit; NND, nearest neighbor distance; p.i., post-infection; SEM, standard error of the mean
Results are the mean ± SEM of 5–6 hippocampi per animal for a total of 5–6 mice per time point
Statistical analyses were performed using a one-way analysis of variance with Tukey’s multiple comparisons test

ISSN: 1742-2094