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Table 3 Morphological parameters of Iba1+/TMEM119+ microglia in both layers of the dorsal hippocampus CA1

From: Microglia are involved in phagocytosis and extracellular digestion during Zika virus encephalitis in young adult immunodeficient mice

Parameters Stratum radiatum Stratum lacunosum-moleculare
Day 0
Mean ± SEM
Day 7 p.i.
Mean ± SEM
Day 10 p.i.
Mean ± SEM
Day 0
Mean ± SEM
Day 7 p.i.
Mean ± SEM
Day 10 p.i.
Mean ± SEM
Cell area (μm2) 239.6 ± 19.0 250.3 ± 15.4 247.9 ± 12.9 146.8 ± 10.7 160.0 ± 93.5 150.2 ± 10.9
Soma area (μm2) 41.08 ± 2.55 48.18 ± 3.40 55.57 ± 3.89* 42.30 ± 0.76 44.06 ± 2.47 51.12 ± 2.22*
Soma perimeter (μm) 25.18 ± 0.92 27.74 ± 1.29 29.62 ± 1.15 (P = 0.0574) 25.35 ± 0.26 26.16 ± 0.92 28.36 ± 0.68*
Arbor area (μm2) 1576.2 ± 90.5 1443.2 ± 98.1 1931.5 ± 132.2# 1204.2 ± 110.0 1046.9 ± 70.8 1351.6 ± 63.8#
Arbor perimeter (μm) 186.0 ± 8.5 187.4 ± 11.1 227.0 ± 11.8 ( P = 0.0505)# 163.4 ± 12.2 158.0 ± 7.7 185.2 ± 4.6
Morphological index (a.u.) 0.028 ± 0.001 0.036 ± 0.001 0.031 ± 0.003 0.041 ± 0.003 0.046 ± 0.002 0.041 ± 0.002
Circularity soma (a.u.) 0.814 ± 0.009 0.794 ± 0.019 0.794 ± 0.010 0.830 ± 0.008 0.810 ± 0.012 0.800 ± 0.011
Solidity soma (a.u.) 0.904 ± 0.005 0.902 ± 0.006 0.899 ± 0.004 0.916 ± 0.003 0.914 ± 0.005 0.914 ± 0.005
Aspect ratio soma (a.u.) 1.653 ± 0.027 1.712 ± 0.053 1.638 ± 0.045 1.611 ± 0.049 1.725 ± 0.057 1.757 ± 0.054
Roundness soma (a.u.) 0.651 ± 0.013 0.626 ± 0.015 0.649 ± 0.015 0.666 ± 0.013 0.620 ± 0.017 0.621 ± 0.013
Circularity arbor (a.u.) 0.581 ± 0.015 0.538 ± 0.024 0.487 ± 0.035 0.572 ± 0.031 0.542 ± 0.017 0.509 ± 0.014
Solidity arbor (a.u.) 0.847 ± 0.007 0.827 ± 0.012 0.804 ± 0.018 0.847 ± 0.013 0.828 ± 0.007 0.810 ± 0.008*
Aspect ratio arbor (a.u.) 1.498 ± 0.047 1.594 ± 0.031 1.478 ± 0.044 1.641 ± 0.047 1.757 ± 0.037 1.567 ± 0.051#
Roundness arbor (a.u.) 0.693 ± 0.020 0.657 ± 0.010 0.706 ± 0.016 0.643 ± 0.017 0.612 ± 0.008 0.670 ± 0.021#
  1. a.u., arbitrary units; p.i., post-infection; SEM, standard error of the mean
  2. Results are the mean ± SEM of 17–21 microglia per animal for a total of 5–6 mice per time point
  3. Statistical analyses were performed using a one-way analysis of variance with Tukey’s multiple comparison test. * P < 0.05 day 10 compared with day 0 (noninfected mice); # P < 0.05 day 10 compared with day 7 p.i.. Both are indicated in bold