Table 3 Morphological parameters of Iba1⁺/TMEM119⁺ microglia in both layers of the dorsal hippocampus CA1

Cell area (μm²)

239.6 ± 19.0

250.3 ± 15.4

247.9 ± 12.9

146.8 ± 10.7

160.0 ± 93.5

150.2 ± 10.9

Soma area (μm²)

41.08 ± 2.55

48.18 ± 3.40

55.57 ± 3.89*

42.30 ± 0.76

44.06 ± 2.47

51.12 ± 2.22*

Soma perimeter (μm)

25.18 ± 0.92

27.74 ± 1.29

29.62 ± 1.15 (P = 0.0574)

25.35 ± 0.26

26.16 ± 0.92

28.36 ± 0.68*

Arbor area (μm²)

1576.2 ± 90.5

1443.2 ± 98.1

1931.5 ± 132.2^#

1204.2 ± 110.0

1046.9 ± 70.8

1351.6 ± 63.8^#

Arbor perimeter (μm)

186.0 ± 8.5

187.4 ± 11.1

227.0 ± 11.8 ( P = 0.0505)^#

163.4 ± 12.2

158.0 ± 7.7

185.2 ± 4.6

Morphological index (a.u.)

0.028 ± 0.001

0.036 ± 0.001

0.031 ± 0.003

0.041 ± 0.003

0.046 ± 0.002

0.041 ± 0.002

Circularity soma (a.u.)

0.814 ± 0.009

0.794 ± 0.019

0.794 ± 0.010

0.830 ± 0.008

0.810 ± 0.012

0.800 ± 0.011

Solidity soma (a.u.)

0.904 ± 0.005

0.902 ± 0.006

0.899 ± 0.004

0.916 ± 0.003

0.914 ± 0.005

0.914 ± 0.005

Aspect ratio soma (a.u.)

1.653 ± 0.027

1.712 ± 0.053

1.638 ± 0.045

1.611 ± 0.049

1.725 ± 0.057

1.757 ± 0.054

Roundness soma (a.u.)

0.651 ± 0.013

0.626 ± 0.015

0.649 ± 0.015

0.666 ± 0.013

0.620 ± 0.017

0.621 ± 0.013

Circularity arbor (a.u.)

0.581 ± 0.015

0.538 ± 0.024

0.487 ± 0.035

0.572 ± 0.031

0.542 ± 0.017

0.509 ± 0.014

Solidity arbor (a.u.)

0.847 ± 0.007

0.827 ± 0.012

0.804 ± 0.018

0.847 ± 0.013

0.828 ± 0.007

0.810 ± 0.008*

Aspect ratio arbor (a.u.)

1.498 ± 0.047

1.594 ± 0.031

1.478 ± 0.044

1.641 ± 0.047

1.757 ± 0.037

1.567 ± 0.051^#

Roundness arbor (a.u.)

0.693 ± 0.020

0.657 ± 0.010

0.706 ± 0.016

0.643 ± 0.017

0.612 ± 0.008

0.670 ± 0.021^#